The Biochemistry Laboratory focuses its research on Carbohydrate Active Enzymes (CAZymes) involved in the biosynthesis, modification and degradation of glycans and glycoconjugates, from fundamental aspects of their mechanism, specificity and regulation to their applications in biocatalysis, synthetic biology and as therapeutic targets in infectious diseases.
Equipment and technologies
The laboratory covers disciplines from Chemistry, Biology and Engineering to address its R&D projects in glycosciences with applications in biotechnology and biomedicine.
- Biocatalysis: development of processes and enzymes for the enzymatic synthesis of value-added products
- Protein engineering: approaches to rational and targeted evaluation
- Bioinformatics and molecular modelling
- Metabolic engineering for metabolite production
- Use of enzymes in the synthesis of bioactive products and pharmaceutical intermediates
- Characterisation of enzyme activity
- Analysis of proteins and protein hydrolyzates. Molecular weight distribution
- Determination of enzyme activity
- Bioanalysis: proteins and nucleic acids
Equipment and technologies
- AKTA and GILSON protein chromatographs for protein purification
- HPLC chromatographs with UV, refractive index and fluorescence detectors
- HPLC chromatograph and MS single Q
- UV/Vis Spectrophotometer
- Spectrofluorimeter
- Microplate readers with UV/Vis, fluorescence
- Differential scanning microcalorimeter, DSC
- Isothermal titration microcalorimeter
- Refrigerated centrifuges
- Automatic titrator
- PCR thermal cyclers
- Cell disruptor
- Sonicator
- Electroporates
- Orbital and CO2 incubators
- Cell culture incubators
- Laminar flow cabinets
- -80ºC Freezer
- Equipos de electroforesis de proteínas en gel
- Liofilizador
- Robot BRAVO
- 7L Brau Bioreactor with probes and control system
